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Characterization of BMSCs and identification of exosomes derived from BMSCs. (A) Morphology of BMSCs of different generations under the microscope. (B) Flow cytometry of the cell surface markers of BMSCs (CD29, CD31, CD44, and CD45). (C) Transmission electron microscopy was used to identify the shape of exosomes. (D) <t>Nanoparticle</t> tracking analysis was utilized to analyze the particle size of exosomes. (E) Detection of exosomal protein markers was performed using western blotting (TSG101, CD9, and CD63).
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nanoparticle tracking analysis (nta; particle metrix zetaview s/n 17–310, germany)  (Particle Metrix)
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Characterization of BMSCs and identification of exosomes derived from BMSCs. (A) Morphology of BMSCs of different generations under the microscope. (B) Flow cytometry of the cell surface markers of BMSCs (CD29, CD31, CD44, and CD45). (C) Transmission electron microscopy was used to identify the shape of exosomes. (D) <t>Nanoparticle</t> tracking analysis was utilized to analyze the particle size of exosomes. (E) Detection of exosomal protein markers was performed using western blotting (TSG101, CD9, and CD63).
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Characterization of BMSCs and identification of exosomes derived from BMSCs. (A) Morphology of BMSCs of different generations under the microscope. (B) Flow cytometry of the cell surface markers of BMSCs (CD29, CD31, CD44, and CD45). (C) Transmission electron microscopy was used to identify the shape of exosomes. (D) Nanoparticle tracking analysis was utilized to analyze the particle size of exosomes. (E) Detection of exosomal protein markers was performed using western blotting (TSG101, CD9, and CD63).

Journal: Frontiers in Immunology

Article Title: Ginsenoside Rh2 Inhibits NLRP3 Inflammasome Activation and Improves Exosomes to Alleviate Hypoxia-Induced Myocardial Injury

doi: 10.3389/fimmu.2022.883946

Figure Lengend Snippet: Characterization of BMSCs and identification of exosomes derived from BMSCs. (A) Morphology of BMSCs of different generations under the microscope. (B) Flow cytometry of the cell surface markers of BMSCs (CD29, CD31, CD44, and CD45). (C) Transmission electron microscopy was used to identify the shape of exosomes. (D) Nanoparticle tracking analysis was utilized to analyze the particle size of exosomes. (E) Detection of exosomal protein markers was performed using western blotting (TSG101, CD9, and CD63).

Article Snippet: The shape and size of exosomes were determined by transmission electron microscopy (TEM, FEI, Tecnai G2 Spirit BioTwin, USA) and nanoparticle tracking analysis (NTA, PARTICLE METRIX, ZetaVIEW S/N 17-310, Germany).

Techniques: Derivative Assay, Microscopy, Flow Cytometry, Transmission Assay, Electron Microscopy, Western Blot